Drosophila: A laboratory manual

2H₂O absolute ethanol acetic acid air-dry aliquots allow antibody aqueous autoclaving beaker CaCl2 cells cells/ml centrifuge tube Cherbas clones comm culture Dechorionate Dehydrate diluted Dissect Dissolve distilled water double-distilled water drop Drosophila Drosophila Ringer's EDTA eggs EGTA embryos ethanol series Euparal final concentration fixative flask formaldehyde formamide freshly prepared g/liter gently Giemsa glass glutaraldehyde glycerol homogenizer humid chamber hybridization hypochlorite Incubate larvae liquid nitrogen MATERIALS medium G mesh METHOD mg/ml microcentrifuge tube micropipette microscope slides microscope slides coverslips mixture mount mountant n-heptane NaCl NaOH needle nuclei orcein overnight paraformaldehyde Pasteur pipette pellet pers pestle Petri dish plastic polytene chromosomes probe Protocol Remove coverslip Resuspend Rinse RNase room temperature salivary glands siliconized squash staining sterile stock solution sucrose supernatant Telephone Telex tissue TMN1 Transfer Tris-HCl Triton twice vitelline membrane volume vortex Wash embryos µg/ml